TgSEC14-LTP1 is a vacuole-secreted SEC14/CRAL-TRIO-like protein required for host DAG flux and PC homeostasis in T. gondii
C-labeling-based lipid flux analysis, and fluorescent lipid uptake assays show that TgSEC14-LTP1 binds phosphatidylcholine (PC) and diacylglycerol (DAG) and is required for efficient host-to-parasite PC and DAG flux and parasite lipid homeostasis. TgSEC14-LTP1 depletion reduces parasite PC and DAG pools, disrupts bulk PC synthesis and parasite biogenesis, delays egress, and ultimately impairs growth. These findings identify TgSEC14-LTP1 as a key Sec14-like PV lumen factor that links lipid binding to host lipid acquisition and PC homeostasis, underscoring the importance of host lipid scavenging for parasite survival.
Authors
- Rosalba Cruz‐Mirón (ORCID: https://orcid.org/0000-0002-1698-0205)
- Cyrille Y. Botté (ORCID: https://orcid.org/0000-0002-2245-536X)
- Mohamed‐Ali Hakimi (ORCID: https://orcid.org/0000-0002-2547-8233)
- Alexandre Bougdour (ORCID: https://orcid.org/0000-0002-5895-0020)
- Jeroen P. J. Saeij (ORCID: https://orcid.org/0000-0003-0289-7109)
- Yoshiki Yamaryo‐Botté (ORCID: https://orcid.org/0000-0002-9349-4411)
- Christophe-Sébastien Arnold (ORCID: https://orcid.org/0000-0002-2392-5837)
- Christopher Swale
- Qinli Liang
Institutions
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Unit of Virus Host Cell Interactions (FR)
- University of California, Davis (US)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Cell Reports
- Published
- 2026-04-21
- DOI
- https://doi.org/10.1016/j.celrep.2026.117299
- Primary Topic
- Cellular transport and secretion
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Foundation for the National Institutes of Health
- Agence Nationale de la Recherche
- MSDAVENIR