Nordihydroguaiaretic acid attenuates TNFα-dependent intercellular adhesion molecule-1 expression in cultured human umbilical vein endothelial cells via targeting TNFα-PI3K-NF-κB-ICAM1 pathway.

Objective: plant, with potent antioxidant and anti-inflammatory traits. This study for the first time elucidates that NDGA attenuates TNFα-dependent ICAM1 expression in the cultured human umbilical vein endothelial cells (HUVECs), by targeting the TNFα-PI3K-NF-κB-ICAM1 pathway. Materials and Methods: Cultured HUVECs were treated with pro-inflammatory and pro-oxidative cytokine TNFα, to induce ICAM1 mRNA level and protein expression on HUVECs cell surface as documented by northern and western blot, respectively. The effect of varying NDGA concentrations was examined on TNFα-stimulated ICAM1 expression and monocyte attachment assay. Results: Pre-TNFα-NDGA treatment of HUVECs moderated TNFα-dependent ICAM1 expression and monocyte attachment on vascular endothelium by inhibiting the PI3K-NF-κB-ICAM1 signaling pathway. Conclusion: impaired cytoplasm to nucleus translocation of pro-oxidative and pro-inflammatory transcription factor NF-κB, moderating the ICAM1 expression and monocyte attachment.