Serological insights of Crimean-Congo haemorrhagic fever virus in podolic cattle bred in Italy
, formerly Crimean-Congo haemorrhagic fever virus (CCHFV) in the serum of podolic cattle from southern Italy. A subset of 20 sera (18 positive and 2 negative) from the initial 670 bovine samples screened by commercial ELISA kit were further evaluated by indirect ELISA and virus neutralization tests. Across the three serological assays, antibodies against CCHFV were detected in a proportion of samples, though the results were not fully concordant and varied according to the assay applied. These findings highlight the need for multiple tests to more accurately assess CCHFV circulation in free-range cattle, particularly in non-endemic areas, and emphasize the importance of careful interpretation when relying on serology alone.
Authors
- Nicola Cavaliere
- Domenico Scaltrito
- Donatella Belluscio (ORCID: https://orcid.org/0000-0002-1051-7949)
- Kerstin Fischer (ORCID: https://orcid.org/0000-0002-8707-3442)
- Julia Holzerland (ORCID: https://orcid.org/0000-0002-0554-8337)
- Antonio Parisi (ORCID: https://orcid.org/0000-0003-2744-1140)
- Maria Assunta Cafiero (ORCID: https://orcid.org/0000-0002-2565-7530)
- Donato Antonio Raele (ORCID: https://orcid.org/0000-0003-2627-0669)
- Caroline Bost (ORCID: https://orcid.org/0000-0001-6523-2054)
- Guido Di Donato
- Domenico Pugliese
- Michela Sordillo
- Lara Caprarella
- Antonella Narducci
- Goffredo Sarti
- Anna Mattea D’Antuono
Institutions
- Friedrich-Loeffler-Institut (DE)
- ASL Roma (IT)
- Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata (IT)
- Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale (IT)
Publication Details
- Journal
- Frontiers in Cellular and Infection Microbiology
- Published
- 2026-04-21
- DOI
- https://doi.org/10.3389/fcimb.2026.1810497
- Primary Topic
- Viral Infections and Vectors
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Center for Selective C-H Functionalization, National Science Foundation