Functional characterization of four glycosyltransferases for biosynthesis of steroidal saponins in medicinal plant Paris polyphylla
exhibit higher expression in leaves and flowers than in stems, indicating distinct tissue-specific patterns. This work identifies key UGTs involved in polyphyllin biosynthesis, enriching the enzymatic toolbox involved in the glucosylation of diosgenin and pennogenin aglycones, and provides a valuable reference for future studies on overproduction of bioactive steroidal glycosides in heterologous hosts.
Authors
- Qixuan Su (ORCID: https://orcid.org/0000-0002-5800-2186)
- Hizar Subthain (ORCID: https://orcid.org/0000-0002-9761-5217)
- Vlada B. Urlacher (ORCID: https://orcid.org/0000-0003-1312-4574)
- Chengjie Zhang (ORCID: https://orcid.org/0000-0001-6885-1678)
- Dandan Tang (ORCID: https://orcid.org/0000-0001-9963-1301)
- Jing Li (ORCID: https://orcid.org/0000-0001-9268-4689)
- Lingli Zhang (ORCID: https://orcid.org/0000-0003-3832-4046)
- S Y Li (ORCID: https://orcid.org/0000-0001-8144-6220)
- Fei Guo
- Lei Du
- Changning Liu
- Luping Chi
- Peng Fang
Institutions
- Qilu University of Technology (CN)
- Shandong University (CN)
- Chinese Academy of Sciences (CN)
- Xishuangbanna Tropical Botanical Garden (CN)
- Qingdao National Laboratory for Marine Science and Technology (CN)
- Heinrich Heine University Düsseldorf (DE)
Publication Details
- Journal
- Synthetic and Systems Biotechnology
- Published
- 2026-04-21
- DOI
- https://doi.org/10.1016/j.synbio.2026.04.002
- Primary Topic
- Phytochemical Studies and Bioactivities
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China