Evaluation of Data-Independent Acquisition-Based Phosphoproteomics Analysis in Mammalian and Bacterial Systems
harvested at different growth phases, phosphosite fold-changes were consistent, whereas statistical significance varied between tools. This work can be informative for workflow selection in DIA phosphoproteomics studies, especially for biological samples with low phosphorylation frequencies.
Authors
- Le Day (ORCID: https://orcid.org/0000-0002-2342-9323)
- Reta Birhanu Kitata (ORCID: https://orcid.org/0000-0002-8973-7679)
- Rosalie Chu (ORCID: https://orcid.org/0000-0001-7428-7647)
- Daniel H. Call (ORCID: https://orcid.org/0009-0007-5530-1598)
- Christoph Grundner (ORCID: https://orcid.org/0000-0003-0596-6798)
- Jon Jacobs (ORCID: https://orcid.org/0000-0003-0557-7338)
- S T Liu
- Issac K. Attah
- Vishant Boradia
- Marina A. Gritsenko
Institutions
- Infectious Disease Research Institute (US)
- Pacific Northwest National Laboratory (US)
- University of Washington (US)
- Physical Sciences (United States) (US)
Publication Details
- Journal
- Journal of Proteome Research
- Published
- 2026-04-21
- DOI
- https://doi.org/10.1021/acs.jproteome.5c01142
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institute of General Medical Sciences
- National Institute of Allergy and Infectious Diseases