Dynamic Switchable and Transient DNA Condensates Driven by Aptamer‐Ligand or Ion‐Nucleobase Bridged Complexes

, and the subsequent ligands MEA/DMSA, result switchable formation/dissociation of the condensates. In addition, crosslinking of Y-shaped DNA reaction modules with palindromic strand, and cooperatively stabilized or destabilized by aptamer/ligand complexes, leads, in the presence of an auxiliary biocatalyst, separating the ligand/aptamer complexes, to the transient evolution/depletion of the condensates. This is exemplified with the adenosine-induced phase-separation of condensates by crosslinking of aptamer subunit-modified Y-shaped modules with palindromic strands cooperatively stabilized by adenosine/aptamer-subunits. In the presence of adenosine deaminase (ADA), transforming adenosine to inosine, the transient, dissipative depletion of the condensates proceeds. Alternatively, crosslinking of DNA Y-shaped modules with palindromic strands conjugated to adenosine aptamer units, leads to the evolution of phase-separated adenosine-responsive microdroplets. In the presence of adenosine and ADA, the transient depletion of the microdroplets and their dynamic temporal reassembly proceeds.