Development of a vaccine based on mRNA assembly of PEDV virus-like particle
. In mice, the mRNA-VLP platform elicited significantly higher systemic IgG, mucosal IgA, and neutralizing antibody titers than an S-only mRNA control or a commercial PEDV/TGEV inactivated vaccine, while expanding both T- and B-cell populations. Maternal immunization of pregnant sows converted these responses into potent systemic immunity that protected suckling piglets against virulent challenge, as evidenced by markedly reduced diarrhea scores, intestinal lesions, viral shedding, and mortality. Collectively, the developed PEDV mRNA-VLP platform offers a highly promising strategy for preventing PEDV infection.
Authors
- Tianyi Zhong (ORCID: https://orcid.org/0000-0002-4323-1277)
- Weilu Guo
- Baochao Fan (ORCID: https://orcid.org/0000-0001-9780-5080)
- Bin Li (ORCID: https://orcid.org/0000-0003-1318-7081)
- Hongqi Shang (ORCID: https://orcid.org/0000-0003-2743-539X)
- Lizhong Wang (ORCID: https://orcid.org/0000-0003-1980-4730)
- Mengdi Yang (ORCID: https://orcid.org/0000-0001-6809-3870)
- Xinmei Geng
- Yongxiang Zhao
- Lixiang Zhao (ORCID: https://orcid.org/0000-0002-1102-082X)
- Shiyu Liu
- Yunchuan Li
- Min Sun
- Mi Hu
- Shanshan Yang
- Xu Song
Institutions
- Jiangsu University (CN)
- Nanjing Agricultural University (CN)
- Soochow University (CN)
- Jilian Technology Group (China) (CN)
- Ministry of Agriculture (CA)
- Taizhou Vocational and Technical College (CN)
- Yangzhou University (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2026-04-21
- DOI
- https://doi.org/10.1128/jvi.02060-25
- Primary Topic
- Animal Virus Infections Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Jiangsu Agricultural Science and Technology Innovation Fund
- National Natural Science Foundation of China
- National Key Research and Development Program of China