Thermodynamically enabled and reaction attuned estimation of metabolic fluxes

, Teraflux achieves high prediction accuracy, preserves biologically essential feasible cycles like the glyoxylate shunt, and prevents implausible metabolic states such as artificial glucose excretion. Furthermore, thermodynamic variability analysis confirms that Teraflux's predictions fundamentally align with physiological limits on intracellular metabolite concentrations. Teraflux provides a robust, computationally efficient tool for obtaining reliable, condition-specific fluxomes.