Bushen Huoxue recipe restores trophoblast proliferation through the PI3K/AKT pathway in recurrent spontaneous abortion

Background: Dysregulation of extravillous trophoblast (EVT) proliferation and invasion has been implicated in the pathogenesis of recurrent spontaneous abortion (RSA). Bushen Huoxue Recipe (BSHXR), a Traditional Chinese Medicine formula for RSA, has notable clinical efficacy; however, the molecular mechanisms by which it ameliorates recurrent spontaneous abortion remain to be elucidated. Objective: This study aimed to identify the key active ingredients in BSHXR and observe the mechanism by which BSHXR alleviates RSA. Methods: . Results: < 0.0001) pathway increased in the BSHXR group. Conclusion: The PI3K/AKT pathway is a potential pathway for RSA intervention. The therapeutic effect of BSHXR is related to the proliferative effect mediated by the PI3K/AKT pathway.