Role of Concentration in Opposing Effects of Anandamide on Nociceptive Synapses versus Non-nociceptive Synapses

have shown that endocannabinoids depress nociceptive (N) sensory cell synapses and potentiate of non-nociceptive pressure (P) sensory cell synapses. In this study, exogenously applied AEA produced depression of N synapses and potentiation of P synapses across the same range of concentrations. However, the results differed when using URB597, a drug that raises AEA by inhibiting fatty acid amine hydrolase (FAAH), the enzyme that metabolizes AEA. Potentiation of P synapses required higher concentrations of URB597 compared with the concentrations needed to elicit depression of N synapses. Interestingly, pairing somatosensory afferent activity with a normally subthreshold concentration of URB597 did elicit potentiation in P synapses. Sensitivity of the nociceptive and non-nociceptive synapses to cannabinoid receptor inhibitors differed when AEA versus UBR597 was applied. This study demonstrates the complexity of AEA-mediated effects on distinct synapse types that may be informative about the basic biology of endocannabinoid modulation of nociception.