Functional Characterization and Mutagenesis Studies of a Microbial-like Diterpene Synthase from Huperzia serrata
and characterize it as a multifunctional diterpene synthase. Through structure-guided mutagenesis, we uncovered the molecular basis of its functional versatility, with the S224A mutation providing a powerful tool for enhancing the yields of all three diterpene skeletons, thereby laying a foundation for future protein engineering and synthetic biology applications.
Authors
- Baofu Xu (ORCID: https://orcid.org/0000-0002-0144-2455)
- Y ZHAO (ORCID: https://orcid.org/0009-0009-4363-3207)
- Bao Chen (ORCID: https://orcid.org/0000-0001-6868-4996)
- F Z Chen
- Ting He
- Xin Li (ORCID: https://orcid.org/0009-0006-9446-0850)
Institutions
- Shandong University of Traditional Chinese Medicine (CN)
- University of Nottingham Ningbo China (CN)
- Chinese Academy of Sciences (CN)
- Shanghai Institute of Materia Medica (CN)
Publication Details
- Journal
- Molecules
- Published
- 2026-04-17
- DOI
- https://doi.org/10.3390/molecules31081329
- Primary Topic
- Plant biochemistry and biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Shandong Province
- National Key Research and Development Program of China