Functional and genomic characterization of LCN2-deficient PC-3 cells reveals insights into prostate cancer progression
Introduction: depletion in PC-3 cells. Methods: -deficient PC-3 clone #1 (PC-3 LCN2-KO#1), developed using CRISPR/Cas9 technology. Short tandem repeat (STR) analyses confirmed the authenticity and lineage of each cell line, while next-generation sequencing coupled with RT-qPCR validation was used to identify differentially expressed genes and any potential genomic changes resulting from the CRISPR/Cas9 editing process. Results and Discussion: -mediated pathways in advanced disease.
Authors
- Dominik G. Grimm (ORCID: https://orcid.org/0000-0003-2085-4591)
- Thomas Haaf (ORCID: https://orcid.org/0000-0002-0737-0763)
- Diandra T. Keller (ORCID: https://orcid.org/0009-0000-2266-3698)
- Phillipp Torkler (ORCID: https://orcid.org/0000-0002-1450-5806)
- Sarah K. Schröder (ORCID: https://orcid.org/0000-0003-2265-3726)
- Kiara Gäberlein (ORCID: https://orcid.org/0009-0008-0158-3224)
- Indrajit Nanda
- Ralf Weiskirchen
- Claus Steinlein
Institutions
- University of Würzburg (DE)
- Weihenstephan-Triesdorf University of Applied Sciences (DE)
- Institute of Geography (KZ)
- Institute of Human Genetics (PL)
- Universitätsklinikum Würzburg (DE)
- Deggendorf Institute of Technology (DE)
- RWTH Aachen University (DE)
Publication Details
- Journal
- Frontiers in Molecular Biosciences
- Published
- 2026-04-20
- DOI
- https://doi.org/10.3389/fmolb.2026.1730948
- Primary Topic
- Acute Kidney Injury Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- RWTH Aachen University