Therapeutic effects of an engineered bionic decoy-integrated versatile immunosuppressive nanosystem based on an in vitro blood-brain barrier model in ischemic stroke

BACKGROUND: Stroke is a class of acute cerebrovascular diseases, among which ischaemic stroke is characterized by high morbidity and high recurrence rate. Due to multiple pathological mechanisms, it is very easy to cause cerebral ischaemia-reperfusion injury (RI). To quickly and effectively regulate the complex and variable cerebral immune microenvironment (IM) and reduce RI, the study aims to use a versatile immunosuppressive nanosystem (VIN) to efficiently remodel the overactivated brain IM and alleviate RI, thereby enhancing the effects of ischemic stroke (IS). METHODS: as a bridge to obtain A151-loaded polydopamine nanoparticles. Finally, it was reconstituted with engineered bionic decoy vesicles using ultrasonic cavitation to prepare a VIN integrated with engineered bionic decoys. RESULTS: NPs) could significantly increase the expression level of CXCR4 receptor. In rats treated by the VIN group, the body weight had increased to near the normal level by day 7, and the infarct area was reduced by 89.4%. The results showed that VIN was able to effectively reduce the volume of cerebral ischemic infarction in rats in long-term treatment, and had a good therapeutic effect on IS. CONCLUSION: The study achieved internal and external synergistic immunosuppressive effects to effectively and safely regulate the excessive IM after reperfusion, which provides a new idea to alleviate ischaemia-RI.