Characterization of Proline-Rich Antimicrobial Peptides with SbmA Transporter-Dependent and Independent Antimicrobial Activity toward <i>Klebsiella pneumoniae</i>
. Bacterial mutants lacking LPS modifications conferring colistin resistance displayed rapid uptake of BODIPY-labeled PR-39 and Bac7 (1-35), whereas mutants lacking the SbmA transporter displayed less uptake of PR-39 than Bac7 (1-35). Overall, these findings highlight peptide charge as a critical determinant of membrane interaction and membrane-mediated uptake while also revealing mechanistic insight into how PrAMPs engage with oligosaccharides.
Authors
- Renee M. Fleeman (ORCID: https://orcid.org/0000-0001-7103-461X)
- Jennifer S. Brodbelt (ORCID: https://orcid.org/0000-0003-3207-0217)
- Hanlin Ren
- RobertL Beckman
- Christina M. DeBarro
- Ridhwana M. Appiah
Institutions
- University of Central Florida (US)
- Organogenesis (United States) (US)
- The University of Texas at Austin (US)
Publication Details
- Journal
- ACS Infectious Diseases
- Published
- 2026-04-20
- DOI
- https://doi.org/10.1021/acsinfecdis.6c00142
- Primary Topic
- Antimicrobial Peptides and Activities
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Welch Foundation
- National Institute of General Medical Sciences
- National Institute of Allergy and Infectious Diseases