Production of bioethanol from certain agricultural waste by fermentation

Biofuels from biomass are seen as a necessary replacement for petroleum due to rise of its price from time to time and its negative impact on the environment. The main objective of this investigation was to isolate yeast strains from sugary rich substrates and test their bioethanol production potential from agricultural wastes. Eighteen yeast strains, varying in color, margin, and cell shape, were successfully isolated from banana, avocado, and mango samples. Initial screening using Yeast Extract Peptone Glucose Broth (YPGB) led to the selection of four high-performing yeast isolates: AMUBa6, AMUBa2, AMUAvo2 and AMUMa3 produced 60.76%, 50.53%, 50.33%, and 47.66%, percent of ethanol respectively. Subsequently, these isolates were tested for bioethanol production using different concentrations of banana, avocado, and mango peels. Isolates AMUBa6 and AMUBa2 demonstrated the highest efficiency, yielding 76.14% and 70.71% ethanol, respectively, from 50 g of agricultural wastes. A significant enhancement in bioethanol yield was observed with the addition of malt to the fermentations medium highly improved the percent of bioethanol yield as compared to the control. The AMUBa6 showed the highest bioethanol percent 85.24 with the 10 g of malt followed by the AMUBa2 81.27. This study has clearly indicated that the possibility of environmental isolated yeast strains converting the agricultural wastes into value added bioethanol. This shows that the double advantages of bioethanol production which in one hand produces a valuable product which will be the potential of energy of the world in the future and also contribute in minimizing the amount of the solid waste from the environment and contribute in the sustainability of the environment.