A SERS-Based Analytical Strategy for Real-Time Quantitative Kinetic Analysis and Inhibitor Screening for Cytochrome P450-Mediated PAH Metabolism
). To our knowledge, this work represents the first instance of SERS for time-resolved monitoring of CYP-mediated metabolism, expanding its capability from static detection to dynamic biochemical analysis. The proposed strategy provides a simple, label-free, and time-resolved approach for enzyme kinetic studies, inhibitor screening, and metabolic analysis.
Authors
- Wei Ji (ORCID: https://orcid.org/0000-0001-6391-9768)
- Yifan Li (ORCID: https://orcid.org/0000-0001-5764-5350)
- Yuting Song (ORCID: https://orcid.org/0000-0003-0073-4226)
- Bo Jiang (ORCID: https://orcid.org/0000-0001-8296-7394)
- Chunyi Wei
- Junbo Li
- Yang Yang
- Yunfei Xie (ORCID: https://orcid.org/0000-0002-2758-9236)
Institutions
- Jiangnan University (CN)
- Culture Resource (EG)
- State Key Laboratory of Food Science and Technology (CN)
- Northeast Forestry University (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-04-20
- DOI
- https://doi.org/10.1021/acs.analchem.6c00025
- Primary Topic
- Spectroscopy Techniques in Biomedical and Chemical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Heilongjiang Province
- Jiangsu Provincial Key Research and Development Program
- Fundamental Research Funds for the Central Universities