Absolute Quantitation of Phenolic Compounds in Olive Oil for Health Claim Recognition

) oil:extract ratio to avoid saturation of the extract in oils with a higher phenolic content and its subsequent determination through LC-MS/MS in multiple reaction monitoring (MRM) mode, the gold standard technique in many application fields because of its analytical features. The optimized method was applied to a set of 100 extra virgin olive oils (EVOOs), and the results obtained were compared with the classic Folin-Ciocalteu method. The comparison between the two methods showed that the classic method is a non-selective method that can be affected by many interferences and that the Folin method underestimates the real phenolic content.