Eco-friendly Indirect Spectrophotometric Method for Estimation of Amiloride Hydrochloride in its Pharmaceutical Preparations Via Eosin Y

A simple, sensitive, and precise indirect spectrophotometric method has been developed for quantifying amiloride hydrochloride (AMD. HCl), both as a pure compound and in its dosage forms (tablets). This work is based on indirect spectrophotometric method of the oxidation of (AMD. HCl) by adding a known amount of oxidizing agent calcium hypochlorite (Ca(OCl)2) in acidic medium. The unreacted amount of Ca(OCl)2 then reacted with a known quantity of the dye eosin Y to shorten its color, and then the absorption of the remaining amount of the dye to form an orange-colored product that can be measured at a wavelength of 518 nm. A thorough investigation was conducted into the optimization of method conditions. Beer's law was followed under ideal circumstances in the concentration range of 2–12 µg/mL, with a relative standard deviation of 2.62%, a recovery of 100.03%, and a determination coefficient (R2) of 0.9992. The limit of quantification (LOQ) was 0.0932 µg/mL, while the limit of detection (LOD) was 0.028 µg/mL. The molar absorptivity coefficient was 1.8856×104 L/mol. cm, which corresponds to Sandall’s sensitivity of 0.0138 µg/cm2. The method was applied to the assay amiloride hydrochloride (AMD. HCl) in tablets, yielding acceptable analytical results. Additionally, a new tool for assessing analytical protocols related to green analytical chemistry attributes had been used in order to assess the environmental impact of the proposed technique. The new instrument is known as the Green Analytical Procedure Index, or GAPI.