Sun-macerated Hypericum perforatum oleates in fixed oils: analytical characterization and comparative wound-healing activity

Background/aim: L. (black seed), is compared. Materials and methods: Fixed oils were characterized by gas chromatography-mass spectrometry, while oleuropein (olive oil) and thymoquinone (black seed oil) were quantified by high-performance liquid chromatography, and sesamin and sesamol (sesame oil) by spectrophotometry. Oleates were prepared using the traditional sun maceration method. Hypericin, hyperforin, and pseudohypericin were quantified by liquid chromatography-mass spectrometry and ultraviolet spectrophotometry. In vitro and in vivo assays were performed to assess the wound-healing activities of both the fixed oils and their respective oleates. Results: All oleates exhibited significantly greater wound-healing activity than the corresponding fixed oils. The most pronounced synergistic effects were observed with the olive oil and sesame oil oleates. Conclusion: oleates and provide a scientific basis for their pharmacological development.