Bacteria-mimetic bioadhesives with multivalent mucoadhesion and drug-compatible delivery

without forming covalent bonds to tissue. Confocal imaging reveals deep tissue penetration (∼80 μm) with markedly enhanced mucin binding and no loss of cytocompatibility. Ex vivo intestinal delivery and in vitro drug release tests demonstrate improved drug transport and tissue exposure compared to carbodiimide-mediated covalent bonding strategy. These findings establish a bacteria-mimetic bioadhesion strategy for tissue repair and drug delivery. Novelty Statement: adhesin to enhance bioadhesion through multivalent, cooperative physical interactions rather than covalent bonding. This strategy significantly toughens adhesion even on chitosan, a polymer already rich in adhesive functional groups. By decoupling bacterial adhesion function from pathogenic risk, this study establishes bacterial adhesion peptides as a safe, modular, and mechanistically distinct foundation for next generation bioadhesives with improved drug compatibility.