Water and Energy Turnover in Chinese Young Adults: A Doubly Labeled Water Study of Metabolic Coupling

BACKGROUND: Accurate estimation of water and energy requirements is fundamental for establishing dietary reference values in young adults. However, evidence integrating objectively measured energy expenditure with detailed water turnover components remains limited in Chinese populations. OBJECTIVES: To quantify water intake, water loss, and energy expenditure in healthy young college students, and to examine how energy metabolism is associated with specific components of water turnover under free-living conditions. METHODS: Twenty-one healthy adults aged 18-25 years participated in a 14-day observational study conducted in Beijing, China. Total energy expenditure (TEE) was measured over 14 days using the doubly labeled water (DLW) method. Physical activity was monitored over 7 consecutive days using a triaxial accelerometer. Water intake was assessed using multiple methods: water from beverages (including plain drinking water and other beverages) was recorded over 7 days using 24 h fluid intake records, while water from food was measured during days 5-7 using weighed food records combined with duplicate portion and direct drying methods. Urinary and fecal water loss were quantified using 24 h collections conducted during days 5-7. Metabolic water production and insensible water losses were estimated using established physiological equations. Multivariable linear regression analyses were conducted to examine associations between energy-related variables and components of water turnover. RESULTS: < 0.01), whereas other water intake components showed no significant associations. CONCLUSIONS: In young adults, energy metabolism appears to be more closely associated with physiologically regulated components of water turnover than with voluntary water intake. These findings suggest a divergence between endogenous and behaviorally regulated pathways of water turnover and highlight the importance of considering component-specific water dynamics when examining hydration and energy balance, although confirmation in larger studies is warranted.