SEC-seq reveals translation-focused metabolic strategies for high IgG productivity in clonal CHO cells

showed clone-specific correlations with IgG, linking glycolysis, mitochondrial respiration, fatty-acid metabolism, and redox to secretion. Together, these results demonstrate that SEC-seq can resolve IgG-coupled metabolic-secretory wiring within and between CHO clones, providing a framework to identify subpopulation and circuit features to engineer or select for improved recombinant protein production.

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Publication Details

Journal
bioRxiv (Cold Spring Harbor Laboratory)
Published
2026-04-17
DOI
https://doi.org/10.64898/2026.04.14.718270
Primary Topic
Viral Infectious Diseases and Gene Expression in Insects
Type
article
Field-Weighted Citation Impact
0.00

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article

SEC-seq reveals translation-focused metabolic strategies for high IgG productivity in clonal CHO cells

Jasmine Tat, J. H. Stevens, Nathan E. Lewis, Fides D. Lay
bioRxiv (Cold Spring Harbor Laboratory)
Viral Infectious Diseases and Gene Expression in Insects
article

SEC-seq reveals translation-focused metabolic strategies for high IgG productivity in clonal CHO cells

Jasmine Tat, J. H. Stevens, Nathan E. Lewis, Fides D. Lay
article en

Abstract

showed clone-specific correlations with IgG, linking glycolysis, mitochondrial respiration, fatty-acid metabolism, and redox to secretion. Together, these results demonstrate that SEC-seq can resolve IgG-coupled metabolic-secretory wiring within and between CHO clones, providing a framework to identify subpopulation and circuit features to engineer or select for improved recombinant protein production.

bioRxiv (Cold Spring Harbor Laboratory)
Amgen (United States) (US), University of Georgia (US), University of California San Diego (US), International Neuromodulation Society (US)
Amgen, National Institute of General Medical Sciences
Openalex Percentile: Top 22%
Viral Infectious Diseases and Gene Expression in Insects
DOIOpen Access PDFOpenAlex
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SEC-seq reveals translation-focused metabolic strategies for high IgG productivity in clonal CHO cells — Jasmine Tat, J. H. Stevens, et al. · bioRxiv (Cold Spring Harbor Laboratory) (2026) | TGRS Research Map | TGRS