Evaluating Neutralizing Antibody Titers by Recombinant Feline Calicivirus with Heterologous Capsid Protein VP1
gene in the GDJM202201 backbone with those from heterologous FCV strains. Notably, these recombinant viruses exhibited reduced viral titers compared to rGDJM-A4822T. Finally, neutralization assays revealed differential neutralizing antibody titers among the recombinant FCVs, with rGDJM-A4822T inducing higher neutralizing antibody titers and cross-neutralizing activity. Collectively, this study establishes an FCV infectious clone that can be used to rescue recombinant viruses carrying heterologous VP1 proteins and to evaluate neutralizing antibody responses.
Authors
- Q Zhang
- Y Zhang
- Jun Luo (ORCID: https://orcid.org/0000-0003-4422-9619)
- Shile Huang (ORCID: https://orcid.org/0000-0002-3239-1072)
- Xiaofeng Guo (ORCID: https://orcid.org/0000-0003-2896-3629)
- Wei Lin (ORCID: https://orcid.org/0000-0002-0455-8752)
- Saisai Li (ORCID: https://orcid.org/0009-0006-1877-3126)
- Yuxing Wang (ORCID: https://orcid.org/0000-0003-3079-5572)
- Hongling He
Institutions
- South China Agricultural University (CN)
- Louisiana State University Health Sciences Center Shreveport (US)
Publication Details
- Journal
- Animals
- Published
- 2026-04-17
- DOI
- https://doi.org/10.3390/ani16081237
- Primary Topic
- Viral gastroenteritis research and epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Guangdong Province
- National Key Research and Development Program of China