High-gradient magnetic separation chip–based small extracellular vesicle isolation for noninvasive subtyping of primary aldosteronism
≥ 0.99), overcoming plasma-derived noise. Clinical validation in 106 patients with PA demonstrated 64.58% sensitivity and 96.88% specificity for sEV-based mutation profiling. The assay identified one aldosterone-producing adenoma (APA) case missed by tissue genotyping, achieving area under the receiver operating characteristic curve (AUC) values of 0.767 ~ 0.852 across mutations. This noninvasive approach could enable curative treatment for millions with undiagnosed PA, advancing precision management of endocrine hypertension through sEV-based liquid biopsy.
Authors
- Shiwei Sun (ORCID: https://orcid.org/0000-0003-4370-0552)
- Mingzhu Yang (ORCID: https://orcid.org/0000-0002-5484-0836)
- Dongxu Qiu (ORCID: https://orcid.org/0000-0001-9311-3968)
- Dong Wang (ORCID: https://orcid.org/0000-0001-5579-8572)
- Y M Li
- Yushi Zhang (ORCID: https://orcid.org/0000-0002-8058-9702)
Institutions
- Beijing Institute of Technology (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking Union Medical College Hospital (CN)
- Beijing Research Institute of Mechanical and Electrical Technology (CN)
Publication Details
- Journal
- Science Advances
- Published
- 2026-04-17
- DOI
- https://doi.org/10.1126/sciadv.aeb4949
- Primary Topic
- Hormonal Regulation and Hypertension
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Chinese Academy of Medical Sciences
- Peking Union Medical College