Multidomain DNA–Protein Mining Reveals Polymorphic Variations in RhlB Enhancing Monorhamnolipid Biosynthesis

. Enhanced catalysis in RhlB3 was attributed to loop mutations (I234V and P238R) that improved substrate binding affinity and domain alignment. This study demonstrates that integrating computational mining with experimental validation can reveal naturally optimized enzyme variants, providing a scalable framework for accelerating enzyme engineering and advancing sustainable biosurfactant production.