Peptide Design for Targeting BTB Domain Homodimerization of BACH2: Complementary <i>In Silico</i> and <i>In Vitro</i> Approaches
using supernatant depletion assay, with peptide 13 showing a trend toward higher affinity. Additionally, the inhibitory effect of peptides on homodimerization of the BACH2 BTB domain was investigated through coimmunoprecipitation studies, revealing peptide 13 as a potential disruptor of homodimerization. Based on these observations, peptide 13 has been identified as a putative BACH2 negative regulator. Furthermore, limitations and future directions are also discussed.
Authors
- Emel Timuçin (ORCID: https://orcid.org/0000-0003-0048-0668)
- Ahmet Can Timuçin (ORCID: https://orcid.org/0000-0002-9483-3593)
- Efe Acar
- Hüveyda Başağa
Institutions
- Gebze Technical University (TR)
- Sabancı Üniversitesi (TR)
- Laboratory of Molecular Genetics (PL)
- Institute of Molecular Genetics (RU)
- Kent Hastanesi (TR)
- Pediatrics and Genetics (US)
- Acıbadem Adana Hospital (TR)
Publication Details
- Journal
- ACS Omega
- Published
- 2026-04-17
- DOI
- https://doi.org/10.1021/acsomega.6c01122
- Primary Topic
- Peptidase Inhibition and Analysis
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Türkiye Bilimsel ve Teknolojik Araştırma Kurumu