A High-Throughput Fluorescence Polarization Assay for Screening Sirtuin Inhibitors
-dependent inhibitors, such as Ex-527 and TM. Finally, our assay showed satisfactory stability and outstanding performance in a pilot library screening. Compared to previous assays, the FP assay uses much less SIRT1-3 enzymes, a feature important for high-throughput library screening. We postulate that the FP assay developed here will accelerate the discovery and development of SIRT1-3 inhibitors.
Authors
- Hening Lin (ORCID: https://orcid.org/0000-0002-0255-2701)
- Kewen Peng (ORCID: https://orcid.org/0000-0003-0791-6372)
- Suryadeep Chakraborty
- Yizhen Jin (ORCID: https://orcid.org/0000-0001-8784-721X)
Institutions
- Howard Hughes Medical Institute (US)
- University of Chicago (US)
- Forma Therapeutics (United States) (US)
Publication Details
- Journal
- ACS Chemical Biology
- Published
- 2026-04-17
- DOI
- https://doi.org/10.1021/acschembio.6c00205
- Primary Topic
- Sirtuins and Resveratrol in Medicine
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Cancer Institute