Dual inhibition of intercellular adhesion molecule-1 and nucleolin reduces RSV infection efficiency in human respiratory organoids
nucleoprotein gene expression, whereas inhibition of EGFR or IGF1R had no observable effect on viral gene expression. Notably, simultaneous suppression of ICAM-1 and NCL resulted in a more substantial reduction in infectious viral titers and RSV F protein expression than inhibition of either protein alone. Our results suggest that both ICAM-1 and NCL may play important roles during RSV infection in human iPSC-derived respiratory organoids.
Authors
- Shimpei Gotoh (ORCID: https://orcid.org/0000-0002-8563-8200)
- Rina Hashimoto (ORCID: https://orcid.org/0000-0002-7174-4563)
- Yuki Kitai (ORCID: https://orcid.org/0000-0003-3271-6807)
- Ayaka Sakamoto (ORCID: https://orcid.org/0000-0001-6922-1280)
- Yoshitaka Nakata
- Kazuo Takayama (ORCID: https://orcid.org/0000-0002-1132-2457)
- Abeer Keshta
- Makoto Takeda
Institutions
- Tokyo Medical University (JP)
- Kyoto University (JP)
- Kyoto International University (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- Molecular Therapy — Nucleic Acids
- Published
- 2026-04-17
- DOI
- https://doi.org/10.1016/j.omtn.2026.102932
- Primary Topic
- Respiratory viral infections research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Agency for Medical Research and Development
- Japan Society for the Promotion of Science
- Japan Science and Technology Agency