Exploiting the Danio rerio model and bioinformatics to explore the effects and molecular networks of Parkinson’s disease drugs in human melanoma

three times higher than that of amantadine and C-DOPA. We also established a zebrafish embryonic human melanoma xenograft model using the SK-MEL-28, FM-55-P, and FM-55-M2 melanoma cell lines. The impact on melanoma cells in a zebrafish xenograft model was comparable to the control for rasagiline and C-DOPA, suggesting limited efficacy against melanoma in this context. Amantadine demonstrated modest dose-dependent effects with a significant decrease in proliferation at higher doses. While these drugs were not expected to have strong anticancer effects, the findings support their safety profile in this context. Our bioinformatics analysis showed molecular associations between the two diseases but also studied drug targets. Further development and investigation are needed to explore the potential effects of PD drugs on human melanoma and to better understand the complex association between both diseases. The use of zebrafish as a xenotransplantation model offers great opportunities to investigate such drugs and their effects on melanoma cells in a living organism.