Systems metabolic engineering for hydroxytyrosol production in <i>Escherichia coli</i>

replenishment strategy, the fermentation process of this strain was optimized, and the retention of HT was significantly improved, laying the foundation for the large-scale production of HT. This study explored the metabolic synthesis pathway and efficient fermentation strategy of HT, providing an innovative fermentation strategy and a practical strategy for the fermentation and production of HT and its related products.