Nanobodies to GPVI as alternative reagents for platelet spreading
. Spreading on Nb2‑3 was inhibited by soluble Nb2 and by Src kinase blockade, confirming GPVI‑dependent signaling, while the non‑blocking nanobody Nb28 had no inhibitory effect. Notably, Nb28 alone supported spreading, demonstrating that ligand‑induced GPVI clustering, rather than binding site location, drives activation. These findings show that Nb2‑3 is a potent and reproducible alternative to CRP‑XL, offering a well‑defined and standardizable reagent for GPVI‑dependent static platelet spreading assays.
Authors
- Steven G. Thomas (ORCID: https://orcid.org/0000-0001-8733-7842)
- Alexandre Slater (ORCID: https://orcid.org/0000-0001-7166-438X)
- Jeremy A. Pike (ORCID: https://orcid.org/0000-0003-4163-0335)
- Natalie S. Poulter (ORCID: https://orcid.org/0000-0002-3187-2130)
- Eleyna M. Martin (ORCID: https://orcid.org/0000-0002-9799-4464)
- Tz-Han Ho
- Stephen P.; id_orcid 0000-0002-7846-7423 Watson
- Hsin-Yun Pan
Institutions
- University of Nottingham (GB)
- Chang Gung University (TW)
- University of Exeter (GB)
- University of Birmingham (GB)
Publication Details
- Journal
- Platelets
- Published
- 2026-04-17
- DOI
- https://doi.org/10.1080/09537104.2026.2653068
- Primary Topic
- Platelet Disorders and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- British Heart Foundation