Spermatogenesis depletion in diploid and triploid yellowtail tetra ( <i>Astyanax altiparanae</i> ) for germ cell transplantation
SSCs/mL with 93% cell viability. The cells were labeled with PKH26 and transplanted via the urogenital papilla into recipients (triploid juvenile males) previously treated with busulfan. PKH26-positive cells were visualized up to 80 days after transplantation. The combination of two sterilization methods appears to be more effective for cell transplantation after the endogenous germ cells have been eliminated from the recipient testes of triploid juveniles.
Authors
- Gabriella Braga Carvalho (ORCID: https://orcid.org/0000-0003-0520-0864)
- Lucia Suárez López
- George Shigueki Yasui (ORCID: https://orcid.org/0000-0002-6105-8308)
- José Augusto Senhorini (ORCID: https://orcid.org/0000-0002-5120-448X)
- Rafaela Manchin Bertolini (ORCID: https://orcid.org/0000-0001-7427-300X)
- Diógenes Henrique de Siqueira-Silva
- Norberto Vianna
- Paulo Monzani
Institutions
- China Three Gorges Corporation (China) (CN)
- Universidade de São Paulo (BR)
- Universidade Federal do Sul e Sudeste do Pará (BR)
- Instituto Chico Mendes de Conservação da Biodiversidade (BR)
- Centro de Investigación Científica y de Educación Superior de Ensenada (MX)
- Universidade Estadual Paulista (Unesp) (BR)
Publication Details
- Journal
- Zygote
- Published
- 2026-04-16
- DOI
- https://doi.org/10.1017/s0967199426100380
- Primary Topic
- Reproductive biology and impacts on aquatic species
- Type
- article
- Field-Weighted Citation Impact
- 0.00