<i>Ex vivo</i> modeling of lung tissue resident antimicrobial responses
model, making it an ideal system for microbiological and immunological research. IMPORTANCE: cultured precision cut lung slices (PCLS) and generate transcriptomic data to assess the global tissue resident host response to viral and bacterial challenges. Our data show (i) that murine PCLS faithfully reflect core responses to viral infection, while missing proinflammatory responses linked to infiltrating immune cells and (ii) that human PCLS show a highly diversified tissue resident immune response to viral infection due to previous exposures of the host to this pathogen. These responses are clearly distinct from antibacterial gene profiles. Our data advertise PCLS as a complex and realistic model to study tissue resident immune responses to microbes in a human system.
Authors
- A Lobrinus
- Mirco Schmolke (ORCID: https://orcid.org/0000-0002-2491-3029)
- Benoît Bédat (ORCID: https://orcid.org/0000-0002-3758-7867)
- Nelli Heikkilä (ORCID: https://orcid.org/0000-0002-8879-1569)
- Hélèna Choltus (ORCID: https://orcid.org/0000-0002-7557-7029)
- Béryl Mazel‐Sanchez (ORCID: https://orcid.org/0000-0001-5446-3017)
- Niccolò Bianchi (ORCID: https://orcid.org/0000-0001-5605-3425)
- Julien Prados (ORCID: https://orcid.org/0000-0002-8546-241X)
- Wolfram Karenovicŝ (ORCID: https://orcid.org/0000-0003-4523-4073)
- Virginie Prendki (ORCID: https://orcid.org/0000-0003-1315-2938)
- Christiane Eberhardt
- Simone Becattini
- Yannick Nissille
- Veronique Serre Beinier
- Nina Leitner
- Fedor Bezrukov
Institutions
- University of Geneva (CH)
- Centre Hospitalier Universitaire Vaudois (CH)
- University Hospital of Geneva (CH)
- National Influenza Center (MN)
- University Hospitals Geneva Medical Center (US)
- Geneva College (US)
- University of Lausanne (CH)
Publication Details
- Journal
- mBio
- Published
- 2026-04-16
- DOI
- https://doi.org/10.1128/mbio.00056-26
- Primary Topic
- interferon and immune responses
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Carigest