Comparative Circulating microRNA Profiling in Dogs with Pyometra and Other Inflammatory Diseases

-value < 0.05), 39 circulating miRNAs were commonly altered in both pyometra and non-reproductive inflammatory diseases compared with healthy controls. A total of 83 miRNAs were differentially expressed in the pyometra group, whereas 4 miRNAs were differentially expressed in the non-reproductive inflammatory disease group. Direct comparison between the two disease groups further identified three circulating miRNAs-cfa-miR-885, cfa-miR-599, and cfa-miR-122-as significantly differentially expressed. These findings suggest that circulating miRNA profiles in dogs with pyometra reflect both systemic inflammation and condition-related molecular variation. Further studies with larger cohorts are warranted to validate the clinical relevance of these candidate circulating miRNAs.