Chemical-Assisted Ligation-qPCR for Locus-Specific Detection of RNA <i>N</i> <sup>4</sup> -Acetylcytidine and 5-Formylcytidine
C sites in rRNA and tRNA from multiple human cell lines. Housekeeping-transcript normalization reduced variability and enabled analysis of NAT10 and ALKBH1 perturbations. Overall, CATAL provides a sequencing-free and ligation-based targeted workflow for site-resolved detection and quantification of RNA modifications that supports mechanistic studies of RNA modification regulation and focused validation in biological samples.
Authors
- Xiang Zhou (ORCID: https://orcid.org/0000-0002-1829-9368)
- Xiumin Liu
- Xiaochen Xue
- Zhiying Wang
- Xinyue Guo
Institutions
- State Administration of Traditional Chinese Medicine of the People's Republic of China (CN)
- Wuhan University (CN)
- Center for Interdisciplinary Studies (IN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-04-16
- DOI
- https://doi.org/10.1021/acs.analchem.6c01071
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China
- Fundamental Research Funds for the Central Universities