Dual-BONCAT reveals distinct subpopulations of anabolically active cells
, we deployed it in a salt marsh sediment community, finding that organisms potentially feeding on plant root sugars were more active during the day, while microbes likely metabolizing sulfur were more active at night. We believe dual-BONCAT will prove useful in many studies, as it illuminates microbial community responses to changing conditions, which has important implications for ecosystem dynamics.
Authors
- Jeffrey J. Marlow (ORCID: https://orcid.org/0000-0003-2858-8806)
- Solange Duhamel (ORCID: https://orcid.org/0000-0002-8435-4695)
- Dylan Mankel (ORCID: https://orcid.org/0000-0002-5574-589X)
- Federica Calabrese (ORCID: https://orcid.org/0000-0002-4660-1405)
- Yasheng Maierhaba
- Claire Momjian
Institutions
- Boston University (US)
- University of Arizona (US)
Publication Details
- Journal
- Applied and Environmental Microbiology
- Published
- 2026-04-16
- DOI
- https://doi.org/10.1128/aem.02391-25
- Primary Topic
- Microbial Community Ecology and Physiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Energy
- National Aeronautics and Space Administration