Modelling the structure of Lsi2, Lsi3, Lsi6 proteins and their role in the intercellular trafficking of silicon from root to shoot in rice

during protein conformational changes. Using quantitative real-time PCR (qRT-PCR), the OsLsi3 gene was expressed most strongly in the root, in both wild and cultivated rice plants. Spectrophotometric analysis revealed significant variations in silicon content across different tissues, with the root displaying the highest intensity around ∼ 415 nm, indicating greater silicon concentration. Together, these structural, expression, and compositional analyses provide new insights into the organisation and potential functional roles of silicon transporters in rice. Our research paves the way for improving secure and sustainable rice yield through the precise manipulation of the selectivity of Lsi2, Lsi3, and Lsi6.