Spatially and temporally comparative proteomics provide insights into dynamic response patterns to PEDV infection

Characterizing host response patterns to porcine epidemic diarrhea virus (PEDV) infection at the protein level is essential for capturing rapid-response events and elucidating the pathogenesis mechanisms of porcine epidemic diarrhea (PED). However, the specificity and dynamicity of the tissue-based proteome in the localized region of infected intestines remain elusive. In this study, we conducted a spatiotemporal comparative proteomics to quantify cellular proteins in jejunal and colon tissues throughout the stages of PEDV infection. By integrating multidimensional host proteomics, we obtained data on the regional heterogeneity associated with susceptibility and dynamic response patterns to PEDV infection, indicating dynamic remodeling of glucose and lipid metabolism and regional variability in the antiviral response. Our approach provides a global view of the effect of PEDV on the piglet intestinal proteome and identifies mechanisms that may support its infection tropism. These discoveries will provide a potent and valid strategy for developing PED intervention strategies.