A Novel RNA-Light Probe for Structured-Illumination Microscopy Super-Resolution Imaging of Nucleolar Ultrastructure and Analysis of Nucleolar Dynamics
enables SIM to monitor nucleolar RNA dynamics in living cells during ferroptosis in real time and to investigate the previously elusive dynamics of nucleolus and nucleolar RNA in fatty liver disease. This study not only establishes a versatile chemical scaffold for next-generation RNA-targeted probes but also opens new avenues for mechanistic exploration of nucleolar biology in both health and disease.
Authors
- Zhongping Zhang (ORCID: https://orcid.org/0000-0002-0991-7611)
- Guangmei Han (ORCID: https://orcid.org/0000-0003-4153-0190)
- Dong Wang (ORCID: https://orcid.org/0000-0002-8139-8502)
- Li Su (ORCID: https://orcid.org/0000-0002-0842-9149)
- Zhihui Feng (ORCID: https://orcid.org/0009-0003-8893-3947)
- Xuhui Wei
- Xiaohe Tian
- Wenzhi Li
- Wei Huang
- Rushan Zhao
- Zijun Shao
Institutions
- Anhui University (CN)
- Sichuan University (CN)
- Anhui University of Traditional Chinese Medicine (CN)
- West China Hospital of Sichuan University (CN)
- National IT industry Promotion Agency (KR)
- National Clinical Research (US)
- Ministry of Education (BD)
- Changhong (China) (CN)
- Anqing Normal University (CN)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2026-04-15
- DOI
- https://doi.org/10.1021/acs.analchem.5c05434
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Anqing Normal University
- Anhui Provincial Department of Education