Isolation, Purification and NMR-Based Structural Elucidation of Sterol Derivatives from Ganoderma resinaceum (Fr.) Karst

This study presents a detailed phytochemical investigation of the medicinal mushroom Ganoderma resinaceum (Fr.) Karst., collected from Mazandaran Province, Iran. Sequential maceration using n-hexane, ethyl acetate (EtOAc), and methanol (MeOH) yielded crude extracts of differing polarity. Chromatographic purification of the EtOAc fraction produced four major sterol derivatives: ergosterol, ergosterol peroxide, 9,11-dehydroergosterol peroxide, and ergosta-7,22-dien-3β-ol. Their structures were confirmed using comprehensive spectroscopic techniques, including 1H NMR, 13C NMR, and DEPT. To the best of our knowledge, this is the first report of these sterol derivatives from Iranian G. resinaceum. The findings highlight the chemical diversity of the species and underscore the relevance of medium-polarity fractions as reservoirs of pharmacologically significant sterols.