Base editing in rice using nuclease‐deactivated CRISPR/Cas‐SF01
Adenine and cytosine base editing using dCas-SF01 and the 35S-CmYLCV-U6 composite promoter successfully introduced targeted base substitutions at multiple loci in rice, with average editing efficiency of 33.3%. Using the protospacer adjacent motif (PAM)-relaxed SF01-IKRR variant enabled base editing using 5'-NTN PAMs in rice.
Authors
- Jian‐Kang Zhu (ORCID: https://orcid.org/0000-0001-5134-731X)
- Mugui Wang (ORCID: https://orcid.org/0000-0003-0484-086X)
- Chunyu Zou (ORCID: https://orcid.org/0009-0005-2125-766X)
- Chuang Wei (ORCID: https://orcid.org/0009-0004-3397-6488)
- Yangqing Xie (ORCID: https://orcid.org/0009-0007-3546-3311)
- Hongzhi Wang (ORCID: https://orcid.org/0009-0008-8532-2063)
Institutions
- Shanxi Agricultural University (CN)
- Southern University of Science and Technology (CN)
- Institute of Crop Sciences (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2026-04-14
- DOI
- https://doi.org/10.1111/jipb.70265
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China