In vitro exploration of the therapeutic potential of mesenchymal stem cell conditioned medium in endometriosis treatment by targeting apoptosis and cellular migration

Background: Endometriosis, a common gynecological disorder involving ectopic endometrial tissue, leads to infertility and chronic pain. Dysregulated apoptosis and abnormal cell migration are key pathological features. Given current treatment limitations, novel strategies like mesenchymal stem cells (MSC) derived conditioned media (CM) are explored due to their rich secretome. Objective: To investigate the effects of CM from healthy menstrual blood-derived MSCs (MenSCs-CM) and human adipose tissue-derived MSCs (ADSCs-CM) on apoptosis and migration in endometriotic MenSCs. Materials and Methods: ratio (real-time polymerase chain reaction) were evaluated. Results: ratio (p = 0.044). Conclusion: CM from ADSCs and MenSCs of healthy women enhances apoptosis and inhibits endometriotic MenSCs migration in vitro, suggesting potential therapeutic strategies for endometriosis.