Paving the way or sharing goods?—Interactions between pairs of Staphylococcus aureus and Pseudomonas aeruginosa sequentially isolated from respiratory samples of patients on mechanical ventilation
Introduction: Bacterial colonization of medical devices is promoting hospital-acquired infections leading to worsening patient outcomes and high costs for society. Sequential bacterial colonization of surfaces may provide altered conditions that benefit pathogens. Methods: provides an altered nutrient environment promoting the growth and settlement of other bacteria. Results: . However, bacterial growth, biofilm formation, ratio of colony forming units, and metabolic profiles were influenced by co-cultivation. The effects varied depending on nutrient content in the medium. Discussion: In general, co-cultures appeared to benefit clinical isolates to a higher degree, compared to reference strains. The results indicate that differences in airway microenvironment between patients may have a large effect on the infection process and which pathogens that persist.
Authors
- Oleksandr Ilchenko (ORCID: https://orcid.org/0000-0001-7343-617X)
- Madeleine Ramstedt (ORCID: https://orcid.org/0000-0003-2646-8501)
- Henrik Antti (ORCID: https://orcid.org/0000-0003-1423-9517)
- Meissiner Gomes-Fernandes (ORCID: https://orcid.org/0000-0002-9454-8848)
- Olena Rzhepishevska (ORCID: https://orcid.org/0000-0002-7912-7447)
- Cristina Prat (ORCID: https://orcid.org/0000-0001-6974-9165)
- Dmytro Sokol
- John Erlingsen
- Alicia Lacoma
Institutions
- Utrecht University (NL)
- Instituto de Salud Carlos III (ES)
- University Medical Center Utrecht (NL)
- Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (ES)
- Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol (ES)
- Hospital Universitari Germans Trias i Pujol (ES)
- Umeå University (SE)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2026-04-15
- DOI
- https://doi.org/10.3389/fmicb.2026.1798383
- Primary Topic
- Bacterial biofilms and quorum sensing
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Kempe Foundation
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Vetenskapsrådet
- Umeå Universitet
- Kempestiftelserna
- Instituto de Salud Carlos III
- European Regional Development Fund