Elucidation of the ty-5 resistance network in tomato against tomato yellow leaf curl virus reveals the involvement of AP2/ERF gene
Introduction: . Methods: near isogenic tomato line and its susceptible recurrent parent at various stages of TYLCV infection. Results: We identified 1,394 differentially expressed genes, including numerous defense-related genes and genes involved in RNA-mediated DNA methylation. In addition, the expression of key regulatory genes such as protein kinases (PKs) and transcription factors (TFs) was also significantly altered. To explore the function of one upregulated TF encoding an APETALA2/Ethylene Responsive Factor (AP2/ERF), tomato plants overexpressing this gene were generated and demonstrated to confer resistance to TYLCV. Discussion: resistance will facilitate the development of tomato germplasm with more durable TYLCV resistance.
Authors
- Jennifer R. Wilson (ORCID: https://orcid.org/0000-0002-6522-1280)
- Samuel F. Hutton (ORCID: https://orcid.org/0000-0002-1635-4861)
- Daniel Hasegawa (ORCID: https://orcid.org/0000-0002-2431-9927)
- Alvin M. Simmons (ORCID: https://orcid.org/0000-0003-0750-0975)
- Chellappan Padmanabhan (ORCID: https://orcid.org/0009-0001-8761-4069)
- Qiyue Ma
- John W. Scott (ORCID: https://orcid.org/0000-0002-1896-9798)
- Reza Shekasteband (ORCID: https://orcid.org/0000-0001-8761-0650)
- Kevin S. Stewart
- Zhangjun Fei
- Md Shamimuzzaman
- Kai-Shu Ling
Institutions
- United States Department of Agriculture (US)
- Cornell University (US)
- Florida Gulf Coast University (US)
- U.S. Vegetable Laboratory (US)
Publication Details
- Journal
- Frontiers in Plant Science
- Published
- 2026-04-15
- DOI
- https://doi.org/10.3389/fpls.2026.1788099
- Primary Topic
- Plant Virus Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Institute of Food and Agriculture