Development of a double-antibody sandwich ELISA for detecting Peste des petits ruminants virus infection

) for PPRV. Of note, the assay exhibited no cross-reactivity with Capripoxvirus (CaPV), Orf virus (ORFV), Lumpy skin disease virus (LSDV), Foot-and-mouth disease virus (FMDV), or Parainfluenza virus 5 (PIV5). The repeatability of the DAS-ELISA was validated with both intra-assay and inter-assay coefficients of variation (CV%) being less than 9%, indicating good repeatability. Compared to the results obtained using the commercial ID Vet kit (ID-Vet, Montpellier, France) for PPRV antigen detection on the 240 tissue samples, the DAS-ELISA showed a concordance rate of 93.75% and a kappa value of 0.86, demonstrating the reliability of the developed method. The results showed that the DAS-ELISA established in this study is a reliable diagnostic method for the PPRV detection. KEY POINTS: • Polyclonal and monoclonal antibodies against PPRV N protein were prepared. • A DAS-ELISA based on mAbs and pAbs for detecting virus infection was developed. • The established DAS-ELISA exhibits excellent sensitivity and specificity.