Comprehensive Genomics and Functional Validation Reveal Evolutionary Insights into Xanthan Gum Biosynthesis and Pathogenesis of <i>Xanthomonas campestris</i> pv. <i>campestris</i>
diversity significantly influences xanthan yield via metabolic feedback without affecting viscosity. Furthermore, we demonstrated that host restriction-modification systems drive exogenous DNA instability by targeting specific methylation motifs. Site-directed mutagenesis of these motifs successfully restored plasmid stability. These findings provide a population-scale framework for Xcc evolution and offer validated strategies for overcoming barriers to genetic engineering.
Authors
- P Chen
- J F Sun
- Y C Zhu (ORCID: https://orcid.org/0000-0002-7306-1053)
- 林宇平
- Shuyao Yang
- 吕松 Lü Song
- M Wang (ORCID: https://orcid.org/0009-0006-2920-0454)
- Zhishuai Li
- Bin Liu
- Liang Yin (ORCID: https://orcid.org/0009-0007-1507-6648)
- Yanhe Ma
Institutions
- Chinese Academy of Sciences (CN)
- Integrative Medicine Institute (US)
- Tianjin Institute of Industrial Biotechnology (CN)
- Hubei University (CN)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-15
- DOI
- https://doi.org/10.1021/acs.jafc.6c00796
- Primary Topic
- Polysaccharides Composition and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Tianjin Municipal Science and Technology Bureau
- National Key Research and Development Program of China