High-Level Difucosyllactose Biosynthesis via Metabolic and Transporter Engineering
to facilitate DFL secretion. Finally, implementing a continuous low-concentration lactose feeding strategy in a 5-L bioreactor achieved a record-breaking DFL titer of 110.3 g/L, the highest production level reported to date. This study demonstrates that integrating systematic metabolic engineering with refined process control provides a robust platform for the industrial production of complex oligosaccharides.
Authors
- Jianmin Wu (ORCID: https://orcid.org/0000-0002-0999-9194)
- Jianming Yao (ORCID: https://orcid.org/0009-0002-1007-0689)
- Caiwen Lao
- Lizhi Zhu
- Lixia Yuan
Institutions
- University of Science and Technology of China (CN)
- Chinese Academy of Sciences (CN)
- Hefei Institutes of Physical Science (CN)
- Czech Academy of Sciences, Institute of Plasma Physics (CZ)
- Centre of Plasma Physics - Institute for Plasma Research (IN)
- Kai Biotech (South Korea) (KR)
Publication Details
- Journal
- Journal of Agricultural and Food Chemistry
- Published
- 2026-04-15
- DOI
- https://doi.org/10.1021/acs.jafc.5c17691
- Primary Topic
- Infant Nutrition and Health
- Type
- article
- Field-Weighted Citation Impact
- 0.00