Disulfidptosis-related genes define a prognostic signature and novel therapeutic targets in Ewing’s sarcoma through transcriptomic analysis and experimental validation

Background: Ewing's sarcoma (ES) is a malignant osseous neoplasm characterized by a dismal prognosis, particularly in its metastatic variant. The significance of disulfidptosis-a newly identified cell death mechanism induced by cystine metabolic imbalance and mitochondrial dysfunction-has yet to be investigated in ES. Thus, the aim of this study was to assess the prognostic significance of disulfidptosis-related genes (DRGs) in this disease. Methods: via siRNA knockdown, assessing proliferation, migration, and invasion. Results: knockdown significantly suppressed ES cell proliferation, migration, and invasion. Conclusion: highlights its oncogenic role and potential as a therapeutic target. Single-cell analysis further elucidates the metabolic heterogeneity and microenvironmental interactions underlying ES progression.