Engineering of the glucose uptake system to increase 2,4-Dihydroxybutyric acid production in Escherichia coli
), under aerobic conditions while suppressing acetate formation. Together, these results demonstrate that GalP-mediated glucose uptake and engineering of the TCA cycle provide a robust metabolic framework for efficient DHB biosynthesis and establish a foundation for further process and pathway development.
Authors
- Anh Thi Nguyen
- Ceren Alkım (ORCID: https://orcid.org/0000-0002-5918-142X)
- Thomas Walther
- Cláudio J.R. Frazão
- Nadine Ihle
Institutions
- Centre National de la Recherche Scientifique (FR)
- Institut National des Sciences Appliquées de Toulouse (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Technische Universität Dresden (DE)
Publication Details
- Journal
- Metabolic Engineering Communications
- Published
- 2026-04-15
- DOI
- https://doi.org/10.1016/j.mec.2026.e00276
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- Deutsche Forschungsgemeinschaft