Enzymatic Charge Switching in Asymmetric Dual-Barrel Nanopipettes for Multiplex and Label-Free Bacterial Detection
. The practical applicability was validated across diverse complex matrices: recovery rates of 97.49-106.94% in milk and drinking water, and 101.37-113.91% in serum (5000-50,000 CFU/mL), demonstrating its potential for diagnosing moderate bacterial infections. The innovation lies in the label-free operation, rapid response, and multiplexing capability of this sensor, which addresses critical limitations in current bacterial monitoring systems and offers a cost-effective on-site pathogen screening platform for applications spanning food safety and clinical diagnostics.
Authors
- Jie Li (ORCID: https://orcid.org/0000-0002-0952-093X)
- R Q Wang
- Chen Wang (ORCID: https://orcid.org/0000-0001-6544-4065)
- Wenyuan Liu (ORCID: https://orcid.org/0000-0002-2536-9416)
- Feng Feng
- Lingfei Han (ORCID: https://orcid.org/0000-0002-1345-5763)
- Mengmeng Hao
Institutions
- Nanjing Normal University (CN)
- China Pharmaceutical University (CN)
- Nanjing Drum Tower Hospital (CN)
- China Academy of Chinese Medical Sciences (CN)
- Guang’anmen Hospital (CN)
Publication Details
- Journal
- Journal of the American Chemical Society
- Published
- 2026-04-14
- DOI
- https://doi.org/10.1021/jacs.5c23220
- Primary Topic
- Nanopore and Nanochannel Transport Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China